Fluorometer

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Background

Significance

Fluorescence quantum yield �determination using relavitive method

One significant use of the fluorometer (or fluorimeter) is the determination of the fluorescence quantum yield. This is done using a relative method based on a reference compound of known quantum yield. The unknown sample and the reference sample are measured at the same excitation wavelengths and measurement conditions. The wavelength-integrated flourescent intensity of both materials are then used in the calculation:

<math>\phi = \phi_{reference} \frac {F_{sample} / A_{sample}} {F_{reference}/ A_{reference}} \left( \frac {n_{sample}} {n_{reference}} \right) ^2\,\!</math>

where

φ is the quantum yield

F= integrated fluorescence intensity

A= absorbance at excitation wavelength

n= refractive index

Operation

External Links

Wikipedia:Fluorescence spectroscopy