Fluorometer
Background
Significance
One significant use of the fluorometer (or fluorimeter) is the determination of the fluorescence quantum yield. This is done using a relative method based on a reference compound of known quantum yield. The unknown sample and the reference sample are measured at the same excitation wavelengths and measurement conditions. The wavelength-integrated flourescent intensity of both materials are then used in the calculation:
- <math>\phi = \phi_{reference} \frac {F_{sample} / A_{sample}} {F_{reference}/ A_{reference}} \left( \frac {n_{sample}} {n_{reference}} \right( ^2\,\!</math>