Difference between revisions of "Fluorometer"
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Cmditradmin (talk | contribs) m (→Significance) |
Cmditradmin (talk | contribs) m (→Significance) |
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One significant use of the fluorometer (or fluorimeter) is the determination of the fluorescence quantum yield. This is done using a relative method based on a reference compound of known quantum yield. The unknown sample and the reference sample are measured at the same excitation wavelengths and measurement conditions. The wavelength-integrated flourescent intensity of both materials are then used in the calculation: | One significant use of the fluorometer (or fluorimeter) is the determination of the fluorescence quantum yield. This is done using a relative method based on a reference compound of known quantum yield. The unknown sample and the reference sample are measured at the same excitation wavelengths and measurement conditions. The wavelength-integrated flourescent intensity of both materials are then used in the calculation: | ||
:<math>\phi = \phi_{reference} \frac {F_{sample} / A_{sample}} {F_{reference}/ A_{reference}} \left( \frac {n_{sample}} {n_{reference}} \right | :<math>\phi = \phi_{reference} \frac {F_{sample} / A_{sample}} {F_{reference}/ A_{reference}} \left( \frac {n_{sample}} {n_{reference}} \right) ^2\,\!</math> | ||
where | |||
φ is the quantum yield | |||
F= integrated fluorescence intensity | |||
A= absorbance at excitation wavelength | |||
n= refractive index | |||
=== Operation === | === Operation === | ||
Revision as of 10:08, 28 February 2011
Background
Significance
One significant use of the fluorometer (or fluorimeter) is the determination of the fluorescence quantum yield. This is done using a relative method based on a reference compound of known quantum yield. The unknown sample and the reference sample are measured at the same excitation wavelengths and measurement conditions. The wavelength-integrated flourescent intensity of both materials are then used in the calculation:
- <math>\phi = \phi_{reference} \frac {F_{sample} / A_{sample}} {F_{reference}/ A_{reference}} \left( \frac {n_{sample}} {n_{reference}} \right) ^2\,\!</math>
where
φ is the quantum yield
F= integrated fluorescence intensity
A= absorbance at excitation wavelength
n= refractive index