Transmission Electron Microscope - Revision history

Cmditradmin: /* Technique */

2011-09-15T18:00:33Z

Technique

← Older revision		Revision as of 11:00, 15 September 2011
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	Script for FEI tecnai f20:		Script for FEI tecnai f20:

	<embed_document width="55%" height="400">/images/8/8f/TEM_script.pdf</embed_document>		<embed_document width="100%" height="400">/images/8/8f/TEM_script.pdf</embed_document>

	=== External Links ===		=== External Links ===

Cmditradmin: /* Technique */

2011-09-15T18:00:10Z

Technique

← Older revision		Revision as of 11:00, 15 September 2011
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	Script for FEI tecnai f20:		Script for FEI tecnai f20:

	<embed_document width="55%" height="400">/images/8/8f/TEM_script.pdf</embed_document>		<embed_document width="55%" height="400">/images/8/8f/TEM_script.pdf</embed_document>

Cmditradmin: /* Technique */

2011-09-15T17:59:53Z

Technique

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Cmditradmin: /* Technique */

2011-09-15T17:59:24Z

Technique

← Older revision		Revision as of 10:59, 15 September 2011
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	Script for FEI tecnai f20:		Script for FEI tecnai f20:
			<embed_document width="55%" height="400">/images/8/8f/TEM_script.pdf</embed_document>



	In a transmission electron microscope an electron beam from the electron gun, passes through an electromagnetic condenser lens , through the sample, through an objective lens and then down to a phosphor screen or CCD camera. The condenser lens controls the size and intensity of the beam hitting the sample. The objective lens controls the magnification.		In a transmission electron microscope an electron beam from the electron gun, passes through an electromagnetic condenser lens , through the sample, through an objective lens and then down to a phosphor screen or CCD camera. The condenser lens controls the size and intensity of the beam hitting the sample. The objective lens controls the magnification.

Cmditradmin: /* Technique */

2011-09-15T17:49:47Z

Technique

← Older revision		Revision as of 10:49, 15 September 2011
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	{{#ev:youtube\|_kpN2jPjRYs}}		{{#ev:youtube\|_kpN2jPjRYs}}

	Script:		Script for FEI tecnai f20:
	~~Text/ Audio~~
	~~“Hello my name is Xiaoxia Gao from the user nano technology from the University of Washington. Today we are going to operate the transmission electron microscope the model is~~ FEI tecnai ~~f20”~~
	In a transmission electron microscope an electron beam from the electron gun, passes through an electromagnetic condenser lens , through the sample, through an objective lens and then down to a phosphor screen or CCD camera. The condenser lens controls the size and intensity of the beam hitting the sample. The objective lens controls the magnification.		In a transmission electron microscope an electron beam from the electron gun, passes through an electromagnetic condenser lens , through the sample, through an objective lens and then down to a phosphor screen or CCD camera. The condenser lens controls the size and intensity of the beam hitting the sample. The objective lens controls the magnification.

	TEM images show internal structures of specimens with magnification up to one million times revealing structures in atomic resolution.		TEM images show internal structures of specimens with magnification up to one million times revealing structures in atomic resolution.
	The first step is to prepare the sample and mount it in the holder.		The first step is to prepare the sample and mount it in the holder.
	Remove the cover tube from the single tilt holder. Insert the tool into the hole and gently lift the clamp.		Remove the cover tube from the single tilt holder. Insert the tool into the hole and gently lift the clamp.
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	Carry the sample with a hand over the end so that arm can’t slide out.		Carry the sample with a hand over the end so that arm can’t slide out.


	~~Text/ Audio~~
	Place the copper cold fingers slowly into a Dewar of liquid nitrogen. Top- off the Dewar with additional liquid nitrogen. This will keep the sample cold.		Place the copper cold fingers slowly into a Dewar of liquid nitrogen. Top- off the Dewar with additional liquid nitrogen. This will keep the sample cold.
	The TEM uses four pieces of software which should be opened in order.		The TEM uses four pieces of software which should be opened in order.
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	Use the “Multifunction knobs (MF)” on either side of the scope to adjust the condenser stigmation in both x and y directions to ensure the beam is round and expands concentrically.		Use the “Multifunction knobs (MF)” on either side of the scope to adjust the condenser stigmation in both x and y directions to ensure the beam is round and expands concentrically.



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	Repeat for beam tilt ppY, and adjust the multifunction X and Y knob until there is one beam image.		Repeat for beam tilt ppY, and adjust the multifunction X and Y knob until there is one beam image.


	Select “Beam shift and center the beam it with the multifunction key and then click Done.		Select “Beam shift and center the beam it with the multifunction key and then click Done.
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	Go back to microscope control software, camera tab, ccd/tv and click “acquire.		Go back to microscope control software, camera tab, ccd/tv and click “acquire.


	To save the image in the digital microscope click the tool icon in the lower right. This brings up a new window.		To save the image in the digital microscope click the tool icon in the lower right. This brings up a new window.
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	The next time you click the “123” icon it will save the current image using the same file prefix with an incremented number.		The next time you click the “123” icon it will save the current image using the same file prefix with an incremented number.

	To prepare another picture go to the microscope control, “camera, and click “Search.		To prepare another picture go to the microscope control, “camera, and click “Search.

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	Lift the phosphor screen and replace the cover.		Lift the phosphor screen and replace the cover.

	Higher magnification is more sensitive to objective stigmation so it is necessary to correct it again.		Higher magnification is more sensitive to objective stigmation so it is necessary to correct it again.

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	At high magnification it is best to use the multifunction dial to move the image around rather than moving the sample.		At high magnification it is best to use the multifunction dial to move the image around rather than moving the sample.

	Adjust the focus step with the dial collar and use the smaller dial to fine-tune the focus. Use smaller steps at higher magnification.		Adjust the focus step with the dial collar and use the smaller dial to fine-tune the focus. Use smaller steps at higher magnification. In phase contrast mode the crystal lattice of the gold particle can be seen.
	In phase contrast mode the crystal lattice of the gold particle can be seen.
	When you are finished go the microscope control, Camera and click Insert to take the camera out.		When you are finished go the microscope control, Camera and click Insert to take the camera out.

	Then go to Setup, click “Column Valves closed” to close the column valves and stop the beam.		Then go to Setup, click “Column Valves closed” to close the column valves and stop the beam.
	Then go to Search click the arrow flap and in the reset section, click “Holder” to put the holder in the original position and angle.
	When the light goes out you can pull the holder out straight until you feel a stop, rotate clockwise until you feel a stop.		Then go to Search click the arrow flap and in the reset section, click “Holder” to put the holder in the original position and angle. When the light goes out you can pull the holder out straight until you feel a stop, rotate clockwise until you feel a stop.

	Then it will be easy to pull the holder out.		Then it will be easy to pull the holder out.

	Place the holder in the tube.		Place the holder in the tube.

	This TEM is able to do energy dispersive x-ray spectroscopy or EDX. It measures energy of x-rays that bounce off the surface of the sample.		This TEM is able to do energy dispersive x-ray spectroscopy or EDX. It measures energy of x-rays that bounce off the surface of the sample.

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	It also asks you to connect the holder cable.		It also asks you to connect the holder cable.
	With the system pumped down and the light off, turn the holder counterclockwise and let the vacuum gently pull the holder in.
	Click “In” and the EDX detector moves in.		With the system pumped down and the light off, turn the holder counterclockwise and let the vacuum gently pull the holder in. Click “In” and the EDX detector moves in.

	This sample contains film layers of silicon and germanium alloys. We can do EDX in TEM mode, but in STEM, since the beam is focused probe about 1 nanometer, we can better locate the position for analysis.		This sample contains film layers of silicon and germanium alloys. We can do EDX in TEM mode, but in STEM, since the beam is focused probe about 1 nanometer, we can better locate the position for analysis.

Cmditradmin: /* Technique */

2011-09-15T17:46:03Z

Technique

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Cmditradmin: /* External Links */

2010-07-06T18:11:04Z

External Links

← Older revision		Revision as of 11:11, 6 July 2010
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	See [[Wikipedia:Transmission_electron_microscope TEM]]		See [[Wikipedia:Transmission_electron_microscope TEM]]

	See [[Wikipedia:Energy-dispersive_X-ray_spectroscopy ~~EDAX~~]]		See [[Wikipedia:Energy-dispersive_X-ray_spectroscopy]]

	[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]		[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]

Cmditradmin: /* External Links */

2010-07-06T18:10:28Z

External Links

← Older revision		Revision as of 11:10, 6 July 2010
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	See [[Wikipedia:Transmission_electron_microscope TEM]]		See [[Wikipedia:Transmission_electron_microscope TEM]]

			See [[Wikipedia:Energy-dispersive_X-ray_spectroscopy EDAX]]
	[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]		[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]

Cmditradmin: /* Technique */

2010-06-29T16:25:42Z

Technique

← Older revision		Revision as of 09:25, 29 June 2010
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	=== Technique ===		=== Technique ===
	Part 1		Part 1
	{{#ev:youtube\|~~myja0zfxl-k~~}}		{{#ev:youtube\|j2A6KeWrqeM}}

	Part 2		Part 2

Cmditradmin: /* External Links */

2010-06-24T22:27:11Z

External Links

← Older revision		Revision as of 15:27, 24 June 2010
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	=== External Links ===		=== External Links ===

	See [[Wikipedia:Transmission_electron_microscope \| TEM]]		See [[Wikipedia:Transmission_electron_microscope TEM]]

	[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]		[http://www.youtube.com/watch?v=fToTFjwUc5M&feature=related Brief Youtube intro]